Molecular Cloning of a Bovine Minisatellite DNA Homologous to Mouse Minisatellite
نویسندگان
چکیده
منابع مشابه
Two hypervariable minisatellite DNA binding proteins.
Hypervariable minisatellite DNA sequences are short, tandemly repeated sequences present at numerous loci in eukaryotes. They stimulate intermolecular homologous recombination up to 13-fold in human cells in culture and may be specific sites for the initiation of recombination in the eukaryotic genome (Wahls, W.P., Wallace, L.J., & Moore, P.D. (1990) Cell 60, 95-103). Reported here is the detec...
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The discovery of an anonymous multiallelic locus in 1980 demonstrated for the first time that the human DNA contains hypervariable regions (Wyman & White, 1980). Eight alleles of a polymorphic locus that was not associated with any known gene were identified during the blot hybridization of total human genomic DNA treated with the restrictase EcoRI with a human DNA fragment (16 tbp in length) i...
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We describe the detection of a ubiquitous DNA-binding protein which appears to interact specifically with tandem-repeated minisatellites. The murine 40 kd protein, which we term Msbp-1, was found to be present in all mouse tissues tested. This protein was bound specifically and with high affinity by double-stranded DNA containing a repeat sequence related to the minisatellite 'core' sequence, a...
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We seek to define sequence-based predictive criteria to identify polymorphic and hypermutable minisatellites in the human genome. Polymorphism of a representative pool of minisatellites, selected from human chromosomes 21 and 22, was experimentally measured by PCR typing in a population of unrelated individuals. Two predictive approaches were tested. One uses simple repeat characteristics (e.g....
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We have recently found that okadaic acid, which shows strong inhibitory activity on protein serine/threonine phosphatases and tumor-promoting activity in vivo and in vitro, induces minisatellite mutation (MSM). Human tumors and chemically induced counterparts in experimental animals are also sometimes associated with MSM. In the present study, we demonstrated minisatellite (MS) instability in s...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 1997
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.68.293